Journal: Molecular medicine reports

Article Title: Disorder of the mevalonate pathway inhibits calcium-induced differentiation of keratinocytes.

doi: 10.3892/mmr.2017.7128

Figure Lengend Snippet: Figure 2. Treatment of KCs with inhibitors of the mevalonate pathway reduced the expression levels of Notch1 and p53. Representative western blot images and quantification of protein expression levels of Notch1 and p53 in KCs treated with PRA, ALD, FTI‑277 and GGTI‑298 inhibitors (A) alone, (B) in combination with two inhibitors or (C) in combination with three or four inhibitors for 48 h, in the presence or absence of Ca2+. Data are expressed as mean ± standard error of three independent experiments. *P<0.01 and #P<0.05 vs. untreated control in the presence of Ca2+. PRA, pravastatin; ALD, alendronate; FTI‑277, farnesyl transferase inhibitor; GGTI‑298, geranylgeranyl transferase inhibitor; KCs, keratinocytes; NEXT, Notch1 extracellular truncation; NCID, Notch1 intracellular domain.

Article Snippet: Anti-K1(mouse monoclonal IgG2a; sc-376224), involucrin (INV; rabbit polyclonal IgG; sc-28557), Notch1 (goat polyclonal IgG; sc-6014), p53 (rabbit polyclonal IgG; sc-6243), phosphorylated (p)-extracellular signal-regulated kinase (ERK; rabbit polyclonal IgG; sc-101761), p-protein kinase B (AKT; rabbit polyclonal IgG; sc-33437) and β-actin (mouse monoclonal IgG1; sc-376421) primary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control

Journal: Nutrients

Article Title: Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness

doi: 10.3390/nu8070439

Figure Lengend Snippet: Primers for reverse transcriptase polymerase chain reaction RT-PCR.

Article Snippet: The membranes were subsequently blocked in 5% non-fat dried milk in Tris-buffered saline (TBS) and were incubated with primary antibodies against cytokeratin 20 (CK20, Abcam, Cambridge, MA, USA), Notch1 (Novus Biologicals, Littletown, CO, USA), β-catenin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and p-GSK3β (Cell Signaling Technology, Danvers, MA, USA) overnight.

Techniques: Reverse Transcription, Polymerase Chain Reaction

Journal: Nutrients

Article Title: Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness

doi: 10.3390/nu8070439

Figure Lengend Snippet: WPE and its bioactive compounds suppress colon CSCs markers, including CD133, CD44, DLK1, and Notch1 as well as Wnt/β-catenin signaling in colon CSCs. CD133 + CD44 + HCT116 cells were treated with varying concentrations of WPE (0, 10, 20, and 40 μg/mL) ( A a , Ba ); or concentrations of (+)-catechin, chlorogenic acid, ellagic acid and gallic acid comparable to 40 μg/mL of WPE ( A b , Bb ) for 6 days. mRNA expressions of CD133, CD44, DLK1 and Notch1 were examined by RT-PCR, and β-actin was used as a loading control ( A ); Protein levels of Notch1, β-catenin and p-GSK3β were examined by Western blot analysis, and α-tubulin was used as a loading control ( B ). Representative blots are shown in left panel and quantified in right panel. The values shown are the means ± SEM. A p -value < 0.05 was considered significant. Ctrl, Control; WPE, walnut phenolic extract; CSCs, cancer stem cells; CAT, (+)-catechin; CGA, chlorogenic acid; EA, ellagic acid; GA, gallic acid.

Article Snippet: The membranes were subsequently blocked in 5% non-fat dried milk in Tris-buffered saline (TBS) and were incubated with primary antibodies against cytokeratin 20 (CK20, Abcam, Cambridge, MA, USA), Notch1 (Novus Biologicals, Littletown, CO, USA), β-catenin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and p-GSK3β (Cell Signaling Technology, Danvers, MA, USA) overnight.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Western Blot

Journal: Nutrients

Article Title: Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness

doi: 10.3390/nu8070439

Figure Lengend Snippet: WPE down-regulates CD133, CD44, DLK1, and Notch1 in human primary cells from colorectal cancer tissue. Primary cancer cells were treated with varying concentrations of WPE (0, 10, 20, and 40 μg/mL) for 3 d. mRNA expressions of CD133, CD44, DLK1 and Notch1 were detected by RT-PCR, and GAPDH was used as a loading control. The values shown are the means ± SEM. A p -value < 0.05 was considered significant. Ctrl, Control.

Article Snippet: The membranes were subsequently blocked in 5% non-fat dried milk in Tris-buffered saline (TBS) and were incubated with primary antibodies against cytokeratin 20 (CK20, Abcam, Cambridge, MA, USA), Notch1 (Novus Biologicals, Littletown, CO, USA), β-catenin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and p-GSK3β (Cell Signaling Technology, Danvers, MA, USA) overnight.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control